45-1 Ramsey Road
Shirley, NY 11967 - Suffolk County
(631) 619-7922
This method is suitable for immunofluorescence on tissue sample.Section preparation: The tissue is sliced to ≤10 µm sections and directly mount to adhesive slides.Air dry for 1 hour. Rinse three times in 1X PBS for 5 min each.Drawing a water repellent circle around sections.Sections are incubated in permeablization solution for 3~5 min at room temperature. NOTE: This step is optimized for intracellular staining.
About IF Protocol - Stick Section:
This method is suitable for immunofluorescence on tissue sample.Section preparation: The tissue is sliced to ≤10 µm sections and directly mount to adhesive slides.Air dry for 1 hour. Rinse three times in 1X PBS for 5 min each.Drawing a water repellent circle around sections.Sections are incubated in permeablization solution for 3~5 min at room temperature. NOTE: This step is optimized for intracellular staining.
Category: Biotechnology
Check the content below for useful Tips & Advice
